Cover of: Analysis of 2,4-D metabolites in higher plants by gas chromatography | Norman Cline Glaze

Analysis of 2,4-D metabolites in higher plants by gas chromatography

  • 47 Pages
  • 1.77 MB
  • English
Herbicides., Plants -- Metabolism., Dichlorophenoxyacetic
Statementby Norman Cline Glaze.
The Physical Object
Paginationiv, 47 leaves :
ID Numbers
Open LibraryOL23530200M

Analysis of 2,4-D metabolites in higher plants by gas chromatography / Pages; Book Title. Analysis of 2,4-D metabolites in higher plants by gas chromatography / By. Glaze, Norman Cline, Publication Details.

Holding Institution. Analysis of 2,4-D metabolites in higher plants by gas chromatography Analysis of 2,4-D metabolites in higher plants by gas chromatography by Glaze, Norman Cline, Publication date Topics Herbicides, Plants, Dichlorophenoxyacetic acid Collection americana Digitizing sponsor University of Florida, George A.

Smathers Libraries ContributorPages: INTRODUCTION Theuseofherbicidesisincreasingrapidlyyearbyyear. 2,4-Dichlorophenoxyaceticacid(2,4-D)isthemostwidelyusedof gh2,4.

Analysis of 2,4-D metabolites in higher plants by gas chromatography By (Dissertant) Norman Cline Glaze, M. (Thesis advisor) Wilcox, T. (Reviewer) Humphreys and S. Gas chromatography mass spectrometry (GC-MS) is an effective system for the analysis of volatile metabolites contributing to vegetable/fruit aromas, plant defense responses, short-chain alcohols, acids, esters and hydrocarbons, and thermally stable compounds.

However, relatively few metabolites are truly volatile and so, many metabolites can. Russian botanist Mikhail Tswett coined the term chromatography in The first analytical use of chromatography was described by James and Martin infor the use of gas chromatography for the analysis of fatty acid mixtures.

Different genetic, physiological, and environmental conditions are likely to alter the metabolic composition of plants. Metabolomics aims to provide a comprehensive and unbiased analysis of all metabolites with a low molecular weight present in a biological sample and is therefore a very powerful tool for characterizing the plant metabolome.

Analysis of Metabolites NMR, LC/MS, CE(/MS) and GC/MS are typically the methods of choice to separate, identify and quantify in the analysis of biological samples. However, most of the metabolites, especially those listed in Table 1, are very polar and do not show any UV absorption, which excludes the use of UV or fluorescence detectors for HPLC.

GC-MS Analysis of Volatile Plant Secondary Metabolites. By Elena E. Stashenko and Jairo René Martínez. Submitted: April 8th Reviewed: October 3rd. Gas Chromatography–Mass Spectrometry-Based Metabolomic Analysis of Wagyu and Holstein Beef by Tomoya Yamada *, Mituru Kamiya and Mikito Higuchi Division of Livestock Feeding and Management, National Agriculture and Food Research Organization, Nasushiobara, Tochigi.

The content of capsaicin, gingerol, and alkylresorcinols in the extract is determined using chemical, chromatographic, and spectrometric analysis. Thin layer chromatography (TLC), gas chromatography coupled with mass spectrometry (GC-MS), high performance liquid chromatography (HPLC), and liquid chromatography tandem mass spectrometry (LC-MS/MS.

Metabolomics: Methods and Protocols examines the state-of-the-art in metabolomic analysis. Leading researchers in the field present protocols for the application of complementary analytical methods, such as gas chromatography-mass spectrometry (GC-MS). Leafy gall is a plant hyperplasia induced upon Rhodococcus fascians infection.

Previously, by genomic and transcriptomic analysis, it has been reported that, at the early stage of symptom development, both primary and secondary metabolisms are modified. The present study is based on the hypothesis that fully developed leafy gall, could represent a potential source of new bioactive compounds.

Comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry analysis of metabolites in fermenting and respiring yeast cells.

Analytical Chemistry. ; 78 (8)– doi: /aco. Metabolites are small molecules (molecular weight metabolism [1]. Metabolomics is a comprehensive analysis of metabolites produced by a biological system or derived from various other external sources such as diet, microbes, or xenobiotic sources [1–3].

Derivatisation of metabolites to make them volatile and amenable to gas chromatography. Trimethylsilylation is the most favoured of the many possible derivatisation treatments that are possible.

Separation by GC, which generally involves highly standardised conditions of gas-flow, temperature programming, and standardised column material to.

Analysis of 2,4-D metabolites in higher plants by gas chromatography / By Norman Cline Glaze Topics: Agronomy, Agronomy thesis Ph. gc-ms analysis of secondary metabolites from the whole plant methanolic extract of drynaria quercifolia (l) j.

smith Article (PDF Available) in Journal of Advanced Applied Scientific Research. Introduction Plants synthesize and emit a large variety of volatile organic compounds, which are involved in plant interactions with the environment.

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1 To date, more than volatiles have been identified from more than 90 plant families, and these contribute approximately 1% of all known plant secondary metabolites. 2 From a physiological standpoint, plant volatiles are mainly involved. However, the total number of metabolites in the plant kingdom is estimated to be between andwhich makes cataloging of all metabolites a challenging task [10,11].

Gas chromatography–mass spectrometry (GC‐MS)–based metabolomics is ideal for identifying and quantitating small‐molecule metabolites (gas chromatography.

Details Analysis of 2,4-D metabolites in higher plants by gas chromatography EPUB

Sigma-Aldrich Online Catalog Product List: 2,4-D metabolites. Metabolomics, the comprehensive analysis of metabolites present in a biological sample, has emerged as the third major path of functional genomics beside mRNA profiling (transcriptomics) and proteomics (Fiehn, ; Sumner et al., ).Metabolomic approaches seek to profile metabolites in a nontargeted way, i.e.

to reliably separate and detect as many metabolites as possible in a single analysis. analysis by thin layer chromatography & a quantitative analysis by standard chemical protocol of secondary metabolites in the pod wall (pericarp) and seeds of the Acacia farnesiana L.

has been studied. Using thin layer chromatography (TLC) different components like Alkaloids, Saponin, Flavonoids, and Terpenoides are isolated & identified. Hitherto, research on plant metabolic profiling using chromatographic techniques coupled to MS technologies for database purposes has been accomplished by gas chromatography (GC)-MS analysis of extracts (Schauer et al., ; Tikunov et al., ).

Description Analysis of 2,4-D metabolites in higher plants by gas chromatography EPUB

GC-MS entails high reproducibility in both chromatography and mass fragmentation patterns. Isolation and identification of metabolites: The biological samples like urine, serum, plasma can be analyzed to identify the metabolites in them.

Identification of compound mixtures. A mixture of compounds like the amino acids, volatile oils, plant extracts can be identified by the use of gas chromatography.

metabolites, cellular and pathway events, the so-called systems biology approach (van Greef et al. Meta-bolomics involves the unbiased quantitative and qualitative analysis of the complete set of metabolites present in cells, body fluids and tissues (the metabolome). Biostatistics (multivariate data analysis; pattern recognition) plays an.

Background: Vinca rosea (Apocynaceae) is one of the most important and high value medicinal plants known for its anticancer alkaloids. It is the iota of the isolated secondary metabolites used in chemotherapy to treat diverse cancers. Several high performance liquid chromatography (HPLC) methods have been developed to quantify the active alkaloids in the plant.

The system was extensively applied to rapid analysis of volatile metabolites from different bacteria: Escherichia coli, Streptococcus pneumoniae, and two strains of Staphylococcus aureus.

A total of metabolite peaks were peculiar to one of the species among metabolite peaks found for all of the bacteria.

Other studies have examined optimal extraction protocols for combined LC-MS and GC-MS analysis, and for plant metabolites by NMR. Detection, dynamic range and quantification The analytical approaches commonly used for metabolomics studies are all capable of measuring broad ranges of molecule classes, but each technique also has limitations.

KEGG PLANT of the KEGG Pathway database has data on secondary metabolites. Data from a variety of plant species such as wheat, rice, Arabidopsis, and pear are found to .Metabolomics is the scientific study of chemical processes involving metabolites, the small molecule substrates, intermediates and products of ically, metabolomics is the "systematic study of the unique chemical fingerprints that specific cellular processes leave behind", the study of their small-molecule metabolite profiles.

The metabolome represents the complete set of. The radish (Raphanus sativus L.) is an important root vegetable crop. In this study, the metabolite profiling analysis of radish roots exposed to lead (Pb) and cadmium (Cd) stresses has been.